Conditions of Use
“Microbiology: A Laboratory Experience” is designed as a comprehensive laboratory manual for undergraduate students in microbiology and allied disciplines. This manual covered topics such as general introduction, microscopy, bacterial culture... read more
“Microbiology: A Laboratory Experience” is designed as a comprehensive laboratory manual for undergraduate students in microbiology and allied disciplines. This manual covered topics such as general introduction, microscopy, bacterial culture methods, staining techniques, biochemical tests, antimicrobial susceptibility tests. It also included a chapter consisting of guided activities focusing on human skin microbiome. In addition, topics such as microbial food safety, epidemiology and public health were also discussed. Although this book covered different topics in microbiology, I felt this book could have provided users with a more comprehensive understanding of microbiology concepts in a more structured and systematic approach. I felt this manual is too focused on a few topics and some of the activities suggested could be more useful for clinical microbiology students.
I did not see any errors in terms of accuracy in the content provided. However, certain generalized statements in certain parts of the manual should be revisited with the current scientific understanding to ensure the accuracy of the content
The contents of the manual are relevant to the field. Certain fundamental concepts such as adjusting the microscope to get better images, difference between magnification and resolution, various biochemical tests and their significance in clinical context are well discussed and useful to students.
I found the manual is simple to follow and use but it is text heavy. Users should spend a significant amount of time reading the content in the text format. This could be improved by adding more figures, images and flow diagrams, etc.
Yes! This manual has used consistent terminology. However, providing definitions of the terminology when first used in the manual would help students to better understand the content
As mentioned, I found this manual requires user to spend reading the text heavy portion of the content. This limits the user to navigate through different sections at will to make them self-explanatory and standalone topics.
Organization of topics in this manual can be improved. Terminologies such as gram staining technique and all are used in the beginning chapters without introducing these concepts earlier. This way of organizing content makes it difficult for the students to follow the content.
Quality of images and figures are not good.
Overall, this laboratory manual is a useful reference for undergraduate students in microbiology and allied fields. The quality of this manual can be improved with better organization, structure, good quality images and figures, flow diagrams.
This laboratory manual is concise and provides a broad scope of topics performed in introductory microbiology laboratories. The lab manual had minimal focus on eukaryotic microbes and viruses. In my opinion, the lab manual could be used for... read more
This laboratory manual is concise and provides a broad scope of topics performed in introductory microbiology laboratories. The lab manual had minimal focus on eukaryotic microbes and viruses. In my opinion, the lab manual could be used for biological science and allied health students (i.e. nursing, physician assistant, etc.). After comparing the table of contents of this lab manual to the American Society of Microbiology's guidelines for an undergraduate microbiology curriculum, most of the competencies are addressed. The lab manual is well organized. It includes easy to understand protocols, applicable tables/diagrams, and data collection worksheets.
The content that I reviewed in the Microbiology lab manual was accurate and unbiased. It included charts, diagrams, pictures, and references.
The content of this lab manual was up-to-date. It begins with biosafety, microscopy, and general techniques used in microbiology lab; moves into staining techniques, growth and metabolism; a section on food safety, public health, and epidemiology; and ends with a writing activity on the Microbe Mythbuster (i.e. research project). In my opinion, these labs can be modified as needed to fit curricular updates or budgetary/bacteria availability constraints.
The lab manual was easy to understand, well organized, and well written. The tone was approachable and relevant for the student. The data collection worksheets and protocols were easy to follow.
The flow of content and formatting was consistent throughout the lab manual. Each lab topic begins with a comprehensible discussion, step by step instructions, and easy to follow worksheets.
The lab manual and table of contents have a nice flow. At the discretion of the instructor, the topics can be rearranged /realigned with minimal disruption to the reader.
The lab manual is well organized. The lab protocols are very clear and easy to follow by the instructor and student.
I accessed the lab manual as a pdf and online. With both versions, the images, charts, and diagrams were undistorted. Color images were clear.
I did not encounter any grammatical or spelling errors.
The lab manual is scientific and focused on microbes that impact all ethnicities, races, and backgrounds. I did not encounter any offensive or insensitive material or cultural biases.
This lab experience covers all the aspects for Microbiology lab needs. It could be used for Biology major students and nursing major students. I like it divided the contents with bacterial shapes for physiology, metabolism and growth... read more
This lab experience covers all the aspects for Microbiology lab needs. It could be used for Biology major students and nursing major students. I like it divided the contents with bacterial shapes for physiology, metabolism and growth characteristics. It is also handy to have the Epidemiology and Public Health and Microbiological Food Safety chapters in this Manuel.
The contents in this lab Manuel are accurate with the tables, pictures, references.
Contents are up-to-date with new technologies used in Mycology!
The text is written in lucid accessible prose with all the tables, pictures, and references.
The text is consistent from introduction to Appendix.
The text is easily to use in each different sections with the clearly division through the contents and the tables, forms.
The lab manual is organized in very clear and logical way for teachers and students to pick up!
The text has clear label and instructions for each topic!
The text is very well written in terms of the language!
The text is from the scientific view and did not show any culturally or personal opinions and impacts.
The Microbes Mythbuster chapter could use a clearer title as other chapters such as Research Project Guide.
This lab book covers a wide array of pertinent topics that are key to an introductory microbiology lab course. It begins with a discussion Biosafety Levels and best practices in the lab, with great links to ASM and CDC resources. Use of the... read more
This lab book covers a wide array of pertinent topics that are key to an introductory microbiology lab course. It begins with a discussion Biosafety Levels and best practices in the lab, with great links to ASM and CDC resources. Use of the microscope is covered in a succinct manner with enough information to be useful but not so much as to be tedious. Bacterial culture methods with opportunities for practical application are plentiful and look like they could be easily adapted to individual course needs. Staining is well covered as is metabolism, physiology and growth. The food safety section utilizes various types of selective and differential media and introduces a practical application of lab skills with the exercise on food contamination. The book includes labs on antibiotic sensitivity, epidemiology and blood smears. The Microbe Mythbuster lab at the end is a great way to incorporate Scientific Literacy assessment into the course. I did not see a glossary although there are several appendices which look to be quite helpful. The reason for the 4 instead of 5 is that while I think the book covers the included topics well, I would like to see some additional sections address microbes other than bacteria a little more thoroughly.
I found the book to be very accurate in terms of the information, photos and drawings. I did not see any errors, typo or otherwise.
The topics included in the text are relevant (Biosafety, microscope use, basic microbiology lab techniques) and will be useful now and into the future. The bacterial metabolism, growth and physiology labs will also be relevant well into the future and easily allow individual courses/instructors to pick and choose the media used based on availability etc. Updates should be easy to incorporate and the Scientific Literacy section has so many options for use, it would be not be difficult to modify it for just about any intro microbiology lab course.
The text is VERY well written, easy to understand and the questions, worksheets and tables to fill in are well organized and very clearly written/setup.
The text is very consistent in content depth and discussion. It explains topics in an understandable way and provides so many practical applications of the material, I think students will relate to the content and exercises very well. This text is very well organized and flows from one topic to the next so nicely.
This text lends itself to reorganization by the instructor, based on course needs. As I perused the text, I could easily navigate the topics and definitely can see where an instructor could realign the order for many of the of the sections to meet the needs of their course.
The course of material through the text is well organized. Steps to exercises are well written and logical in sequence.
All images drawings and diagrams are well presented. Color photos are clear, no blurriness. Charts are organized and fit well into the material.
I did not see any grammatical errors.
I saw no incidences of any topics that would be considered culturally insensitive. I would consider all of the topics covered in the text to be inclusive as the microbes and topics chosen would be relevant to all races, ethnicities and backgrounds.
Table of Contents
- Biosafety Practices and Procedures for the Microbiology Laboratory
- The Microscopic World
- Bacteriological Culture Methods
- The Human Skin Microbiome Project
- Differential Staining Techniques
- Metabolism, Physiology, and Growth Characteristics of Cocci
- Metabolism, Physiology, and Growth Characteristics of Bacilli
- Microbiological Food Safety
- The War on Germs
- Epidemiology and Public Health
- Blood: The Good, the Bad, and the Ugly
- Microbe Mythbuster
About the Book
As a group of organisms that are too small to see and best known for being agents of disease and death, microbes are not always appreciated for the numerous supportive and positive contributions they make to the living world. Designed to support a course in microbiology, Microbiology: A Laboratory Experience permits a glimpse into both the good and the bad in the microscopic world. The laboratory experiences are designed to engage and support student interest in microbiology as a topic, field of study, and career.
This text provides a series of laboratory exercises compatible with a one-semester undergraduate microbiology or bacteriology course with a three- or four-hour lab period that meets once or twice a week. The design of the lab manual conforms to the American Society for Microbiology curriculum guidelines and takes a ground-up approach — beginning with an introduction to biosafety and containment practices and how to work with biological hazards. From there the course moves to basic but essential microscopy skills, aseptic technique and culture methods, and builds to include more advanced lab techniques. The exercises incorporate a semester-long investigative laboratory project designed to promote the sense of discovery and encourage student engagement. The curriculum is rigorous but manageable for a single semester and incorporates best practices in biology education.
About the Contributors
Holly Ahern is an award-winning professor of microbiology at SUNY Adirondack in Queensbury, NY with over 30 years of teaching and research experience in molecular biology and microbiology. She has written nationally published laboratory textbooks in cell and molecular biology, and is the author of scientific articles published in both scholarly and trade journals. Ahern was named a National Science Foundation American Society for Microbiology (ASM) Biology Scholar in 2008, completing the Biology Scholar Transitions Residency program in 2011. Her goal is to capture student interest in microbiology and science by immersing them in research.